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KMID : 0811719990030040439
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Korean Journal of Physiology & Pharmacology
1999 Volume.3 No. 4 p.439 ~ p.445
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Effect of Imipramine on Calcium Utilization of Single Cells Isolated from Canine Detrusor
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Ho-Shik Shim
Hyoung-Chul Choi/Young-Sook Jeong/Jong-Ho Kim/Kwang-Youn Lee/Uy-Dong Sohn/Jeoung-Hee Ha
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Abstract
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This study is to investigate the mechanism of inhibitory effect of imipramine on the calcium utilization in single cells isolated from canine detrusor. 2 mm thick smooth muscle chops were incubated in 0.12% collagenase solution at 36circC, and aerated with 95% O2/5 and then cell suspension was examined. Acetylcholine (ACh) evoked a concentration-dependent contraction of the isolated detrusor cells in normal physiologic salt solution (PSS), and the ACh-induced contraction was significantly inhibited by imipramine. In Ca2+?free PSS, ACh-induced contraction was less than those in normal PSS and it was not affected by the pretreatment with imipramine. Ca2+?induced contraction in Ca2+?free PSS was supressed by imipramine, but addition of A 23187, a calcium ionophore, overcomed the inhibitory effect of imipramine. High potassium-depolarization (40 mM KCl) evoked cell contraction, which was inhibited by imipramine. Caffeine, a releasing agent of the stored Ca2+ from sarcoplasmic reticulum, evoked a contraction of the cells that was not blocked by the pretreatment with imipramine. These results suggest that imipramine inhibits the influx of calcium in the detrusor cells through both the receptor-operated- and voltage-gated-calcium channels, but does not affect the release of calcium from intracellular storage site.
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KEYWORD
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Imipramine, Calcium, Single cells, Canine detrusor,
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